Live-or-Dye 650/720 Dye Profile

Biotium™

633-640

Laser Line

650

Excitation Max

720

Emission Max

About Live-or-Dye 650/720


Biotium's Live-or-Dye™ dyes are amine-reactive and used for live/dead cell discrimination in flow cytometry. These probes pass through the permeable cell membrane of dead cells to stain intracellular amine groups. However, the probe cannot pass through the intact membranes of live cells. Additionally, these dyes are very stable and can withstand fixation, permeabilization, and will not transfer between cells. Live-or-Dye™ 650/720 has an excitation peak at 650 nm and an emission peak at 720 nm. In flow cytometry, the 633nm laser is typically used for excitation.



Spectra