Live-or-Dye 700/745 Dye Profile

Biotium™

633-640, 785

Laser Line

700

Excitation Max

725

Emission Max

About Live-or-Dye 700/745


Biotium's Live-or-Dye™ dyes are amine-reactive and used for live/dead cell discrimination in flow cytometry. These probes pass through the permeable cell membrane of dead cells to stain intracellular amine groups. However, the probe cannot pass through the intact membranes of live cells. Additionally, these dyes are very stable and can withstand fixation, permeabilization, and will not transfer between cells. Live-or-Dye™ 700/745 has an excitation peak at 700 nm and an emission peak at 725 nm. In flow cytometry, the 633nm laser is typically used for excitation.



Spectra