X-Rhod-1 (Ca2+ bound)
X-Rhod-1 (Ca2+ bound) (Ex-Max 578 nm/Em-Max 600 nm)
X-Rhod-1, AM is a calcium indicator used in fluorescence microscopy applications. It has an excitation peak at 578 nm and an emission peak at 600 nm when bound to calcium. However, this indicator is a BAPTA-derivative and measurement can be affected by the presence of Mn^2+, Zn ^2+ and Pb^2+.
Excitation & emission spectra
Key facts
- Excitation maximum
- 578 nm
- Emission maximum
- 600 nm
- Optimal laser line
- 561
- Optimal filter
- 610/20
X-Rhod-1 (Ca2+ bound) has an excitation maximum at 578 nm and an emission maximum at 600 nm, a Stokes shift of 22 nm. It is optimally excited by the 561 laser line.
Frequently asked questions
- What is X-Rhod-1 (Ca2+ bound)?
- X-Rhod-1, AM is a calcium indicator used in fluorescence microscopy applications. It has an excitation peak at 578 nm and an emission peak at 600 nm when bound to calcium. However, this indicator is a BAPTA-derivative and measurement can be affected by the presence of Mn^2+, Zn ^2+ and Pb^2+.
- What laser excites X-Rhod-1 (Ca2+ bound)?
- X-Rhod-1 (Ca2+ bound) is optimally excited by the 561 laser line. Its excitation maximum is 578 nm.
- What filter should I use to detect X-Rhod-1 (Ca2+ bound)?
- A 610/20 filter captures the peak of the X-Rhod-1 (Ca2+ bound) emission near 600 nm with minimal spillover.