About MitoTracker Orange

MitoTracker Orange CMTMRos from Thermo Fisher Scientific is a potential-dependent mitochondrial localization probe with an excitation peak at 550 nm and an emission peak at 573 nm. It is recommended for fluorescence microscopy and live-cell imaging. It is retained with fixation using paraformaldehyde. This probe is also available in a reduced version that can aid in reducing background signal. The reduced version, MitoTracker Orange CM-H2TMRos, becomes more intensely fluorescent when it is oxidzed at the mitochondrial membrane.