LysoBrite Deep Red
LysoBrite Deep Red (Ex-Max 595 nm/Em-Max 615 nm)
LysoBrite Deep Red from AAT Bioquest is a cell-permeant alkaline probe that localizes to acidic compartments inside the cell, such as endosomes and lysosomes. These types of probes are designed for live-cell imaging and do not typically survive fixation and permeabilization. LysoBrite Deep Red has an excitation peak at 595 nm and an emission peak at 615 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 595 nm
- Emission maximum
- 615 nm
- Optimal laser line
- 594
- Optimal filter
- 610/20
LysoBrite Deep Red has an excitation maximum at 595 nm and an emission maximum at 615 nm, a Stokes shift of 20 nm. It is optimally excited by the 594 laser line.
Frequently asked questions
- What is LysoBrite Deep Red?
- LysoBrite Deep Red from AAT Bioquest is a cell-permeant alkaline probe that localizes to acidic compartments inside the cell, such as endosomes and lysosomes. These types of probes are designed for live-cell imaging and do not typically survive fixation and permeabilization. LysoBrite Deep Red has an excitation peak at 595 nm and an emission peak at 615 nm.
- What laser excites LysoBrite Deep Red?
- LysoBrite Deep Red is optimally excited by the 594 laser line. Its excitation maximum is 595 nm.
- What filter should I use to detect LysoBrite Deep Red?
- A 610/20 filter captures the peak of the LysoBrite Deep Red emission near 615 nm with minimal spillover.