About ATTO 542

ATTO 542 from ATTO-TEC has an excitation peak at 542 nm and an emission peak at 652 nm. ATTO 542 is a new fluorescent label related to ATTO 532. The new dye is very hydrophilic and shows excellent water solubility. Characteristic features of the label are strong absorption and high photo-stability. The dye shows a very high fluorescence quantum yield even after coupling to a biomolecule. ATTO 542 is very suitable for oligonucleotide labeling, single-molecule detection applications and high-resolution microscopy (PALM, dSTORM, STED etc.). Additionally the dye strongly qualifies to be applied in flow cytometry (FACS), fluorescence in-situ hybridization (FISH) and many more. After coupling to a substrate the dye carries a net electrical charge of - 3.