Biotin CF555 (Hyb8, Mouse) - Reactivity: All Species
Product Details
| Description | This antibody recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This MAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors. | |
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| Conjugate | CF555 | |
| Clone | Hyb8 | |
| Target Species | All Species | |
| Applications | IHC, IHC-P | |
| Supplier | Biotium | |
| Catalog # | Sign in to view product details, citations, and spectra | |
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About CF555
CF®555 from Biotium is a fluorophore with an excitation peak at 555 nm and an emission peak at 565 nm. It can be useful in both fluorescence microscopy and flow cytometry. It is spectrally similar to Alexa Fluor™ 555, ATTO 550 and DyLight™ 550. In Flow Cytometry, R-PE is a brighter alternative to CF®555. In microscopy, it has been validated for use in super-resolution imaging by STORM.
CF®555 from Biotium is a fluorophore with an excitation peak at 555 nm and an emission peak at 565 nm. It can be useful in both fluorescence microscopy and flow cytometry. It is spectrally similar to Alexa Fluor™ 555, ATTO 550 and DyLight™ 550. In Flow Cytometry, R-PE is a brighter alternative to CF®555. In microscopy, it has been validated for use in super-resolution imaging by STORM.
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Validation References
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